P-246 Exposure to oral contraceptives alters human endometrial stem cells culture media metabolomics

Abstract Study question Can oral contraceptives (OCs) influence the metabolism of endometrial stem cells (EnMSC)? Summary answer Altered metabolomic profiling in culture media may be an effect OC hormonal properties on EnMSC metabolism. What is known already The reconstitution tissue relies presence EnMSC, which reside perivascular space endometrium. This process can affected by OCs decreasing secretory potential glands. In case high dose OCs, hyperplasia vessels and stroma atrophy glands occur. However, precise production EnMSCs remains unknown. design, size, duration was a prospective study that included samples menstrual blood from 5 volunteers. conducted for one year received approval Ethics Research Committee. Written informed consent obtained all participants. Participants/materials, setting, methods Five women with regular cycles were two groups according to use OCs: (n = 2) non-OC 3). Samples collected cup second day cycle. medium each passage. Quantitative metabolomics performed multiple reaction monitoring, followed liquid chromatography mass spectrometry. Data analyzed PLS-DA T-test. Potential biomarkers assessed ROC curve. Main results role chance characterized as mesenchymal range 47,4% 85% positive markers. From 186 metabolites quantified groups, 15 proposed discrimination between PLS-DA, also demonstrated total separation statistical model. curve showed 4 out presented more than 80% sensitivity. These are Alanine, Phosphatidylcholine (PC) aa C30:0, Glycine PC C32:2, whose concentrations higher group group. Students’ T-Test analysis confirmed significant p values. have been associated several metabolic processes, including energy alteration molecular pathways related thrombosis cancer. Limitations, reasons caution small number participants further studies necessary confirm findings. Wider implications findings could affect characteristics crucial reproductive success, such receptivity. provides preliminary insight into response based specific metabolite signatures, contribute development future new therapies. Trial registration Foundation Coordination Higher Education Personnel (CAPES – Brazil).